| RASĀYAN - Journal Of Chemistry | Vol.2, No.2, 257-542 (2009) |
ANALYSIS OF TREHALOSE IN ARABIDOPSIS THALIANA L. IN ADDITION, HELPFUL AT ALL STAGES OF HD
Authors: Sahar Rezvani* and Shahab Shariaty
1Young Researchers Club Rasht Branch,Department of Plant Biology Islamic Azad,University Rasht Branch, Guilan, Iran; 2Young Researchers Club Rasht Branch, Department of Chemistry Islamic Azad University,Rasht Branch, Rasht, Guilan, Iran;* Email: Sahar_Rezvani2056@yahoo.com
Trehalose is a disaccharide that occurs naturally in insects, plants, fungi, and bacteria. The major dietary source is mushrooms. Trehalose is used in bakery goods, beverages, confectionery, fruit jam, breakfast cereals, rice, and noodles as a texturizer, stabilizer, humectant, or formulation aid with a low sweetening intensity. Trehalose is a naturally occurring disaccharide with known protein and membrane stabilizing capability. Although trehalose absorption in humans has not been well studied, a small fraction (0.5%) is likely to be absorbed by passive diffusion, as has been demonstrated for other dissacharides 26. In mammalian cell culture, trehalose is moved from the extracellular to intracellular compartment via a fluid phase endocytotic mechanism, and is dependent on extracellular concentration 11. Because of these unique chemical properties, this molecule has been the focus of study in several neurodegenerative diseases, which are associated with the misfolding of disease-specific proteins. Arabidopsis thaliana is a small flowering plant that is widely used as a model organism in plant biology. Arabidopsis is a member of the mustard (Brassicaceae) family, which includes cultivated species such as cabbage and radish. Arabidopsis is not of major agronomic significance, but it offers important advantages for basic research in genetics and molecular biology. In this study, one Arabidopsis thaliana L. in the stress conditions, growthing was analysed for the presence of trehalose. Using as anion-exchange high performance liquid chromatography (HPLC) analysis, trehalose was highest in flower and root. Furthermore, trehalose metabolizing enzymes, trehalose-6-phosphate synthase (TPS) and trehalase enzyme activities were measured in flower and root. TPS activity sharply increased under stress conditions growth.
| Page No. : 267-270 | Received: 10 June 2009 Accepted: 3 July 2009 RJC-398 |
