OPTIMIZED AND VALIDATED REVERSE PHASE HPLC METHOD FOR THE DETERMINATION OF TRANDOLAPRIL IN BULK AND FORMULATIONS

Authors: C.Rambabu1,*, G.Ramu1, A.Biksham Babu1 and S.Venkata Rao1

*E-mail: rbchintala@gmail.com

A simple, sensitive and accurate reverse phase liquid chromatographic method has been developed to determine the amount of active pharmaceutical ingredient trandolapril and applied for the analysis pharmaceutical formulation. Chromatographic determination is performed on a Hypersil gold C18(100mm, 4.6mm-ID; 5µm) with  mobile phase consisting of 50%  buffer and 50% acetonitrile v/v, and flow rate at 1.0 ml/min, keeping the column in thermostat at a constant temperature of 30^oC. The response of the instrument is recorded at maximum wavelength of absorption 215nm. The standard curve is linear over a concentration range of 25.0-150μg/ml (r²=0.9999).  The limit of detection and limit of quantification are calculated and found to be 1.149 and 3.832μg/ml. The developed method is validated statistically. The low percent of relative standard deviation (0.297) and high percent of recovery of the drug (99.78%-100.23) indicate that the developed method is precise and accurate.

Keywords : Trandolapril, Liquid Chromatography, Mobile Phase, Chromatogram, Limit of Detection, Assay.