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| RASĀYAN - Journal Of Chemistry | RJC, Vol.4, No.3, 498-700(2011) |
DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF LACOSAMIDE IN BULK AND ITS PHARMACEUTICAL FORMULATION
Authors: V.Kalyan Chakravarthy* and D.Gowri Sankar
*E-mail: Kalyan224@rediffmail.com
An isocratic reverse phase liquid chromatography (RP-LC) method has been developed and subsequently validated for the determination of Lacosamide in Bulk and its pharmaceutical formulation. Separation was achieved with a Develosil ODS HG-5 ((Make: Nomura chemicals (Japan); 150 mmx4.6 mm I.D; particle size 5 μm)) Column and Sodium di-hydrogen phosphate monohydrate buffer (pH adjusted to 3.0 with diluted orthophosphoric acid): Acetonitrile (700:300) v/v as eluent at flow rate 1.0 mL/min and the Column temperature was 40°C. UV detection was performed at 210nm and sample temperature was maintained at 5°C. The method is simple, rapid, and selective. The described method of Lacosamide is linear over a range of 3.996 μg/mL to 47.952 μg/mL. The method precision for the determination of assay was below 2.0%RSD. The percentage recoveries of active pharmaceutical ingredient (API) from dosage forms ranged from 100.0 to 101.3%. The method is useful in the quality control of Bulk and pharmaceutical formulations.
| Page No. : 666-672 | RJC-811/2011 |
